Wyniki wyszukiwania dla: Marco Rito‐Palomares

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Primary recovery strategies of low‐molecular‐weight toxins from Crotalus molossus nigrescens and Crotalus atrox using aqueous two‐phase and three‐phase partition systems

Calef Sánchez‐Trasviña, David Melendez‐Martínez, Emiliano González‐Castañón, Armando Herrera‐Ortuño, więcej

Journal of Chemical Technology & Biotechnology > 99 > 4 > 769 - 779

BACKGROUND Rattlesnake venoms are a complex source of different toxin families with biotechnological and biomedical applications. However, the complexity of the sample and the low abundance of some toxins can restrict their study and applications. Toxin isolation usually requires multiple chromatographic steps, implying elevated operative costs and processing times. This raises the need for recovery...

artykuł

Characterization of polymer‐polymer aqueous two‐phase system droplets for 3D culture future applications

Karolina Chairez‐Cantu, Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 98 > 8 > 1888 - 1895

BACKGROUND Cell‐based high‐throughput platforms using aqueous two‐phase systems (ATPS) have led to the construction of 3D cultures using ATPS in which stem cells are confined into polymer–polymer droplets, giving rise to viable spheroids for their further proliferation and differentiation. This work aims to characterize polymer–polymer ATPS droplet generation based on diameter size, morphology and...

artykuł

Laccase–luminol chemiluminescence system: an investigation of substrate inhibition

Calef Sánchez‐Trasviña, José Daniel Galindo‐Estrada, Raunel Tinoco‐Valencia, Leobardo Serrano‐Carreón, więcej

Luminescence > 38 > 3 > 341 - 349

Chemiluminescence (CL) reactions are widely used for the detection and quantification of many types of analytes. Laccase has previously been proposed in CL reactions; however, its light emission behaviour has not been characterized. This study was conducted to characterize the laccase–luminol system, determine its kinetic parameters, and analyze the effects of protein and OHˉ concentration on the...

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TMB vs ABTS: comparison of multi‐enzyme‐based approaches for the colorimetric quantification of salivary glucose

Alonso Ornelas‐González, Marco Rito‐Palomares, Mirna González‐González

Journal of Chemical Technology & Biotechnology > 97 > 10 > 2720 - 2727

BACKGROUND Diabetes is one of the fastest‐growing health problems of recent times. Its diagnosis, monitoring and control are carried out mainly by glucose quantification in blood samples, which can be a traumatic and painful procedure for many patients. Saliva is an emerging diagnostic biofluid for minimally invasive disease diagnosis. Its collection can be performed through a simple, noninvasive...

artykuł

Development of a simple and flexible enzyme‐based platform for the colorimetric detection of multiple biomarkers in non‐conventional biofluids

Alonso Ornelas‐González, Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 97 > 8 > 1959 - 1965

Background Enzyme‐based colorimetric systems are inexpensive, simple, adaptable and sensitive methods that allow specific quantification of a substrate. In the clinical field, their use has been the basis for the development of equipment and devices that today are key for disease detection. The objective of this project was to demonstrate the flexibility of a previously developed multi‐enzyme system...

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Special Issue: biotechnology advances in Latin America

Marco Rito‐Palomares, Guillermo Quijano

Journal of Chemical Technology & Biotechnology > 97 > 6 > 1351 - 1352

artykuł

Aqueous two‐phase systems in Latin America: perspective and future trends

Mirna González‐González, Guillermo Picó, Álvaro Silva Lima, Cassamo Ussemane Mussagy, więcej

Journal of Chemical Technology & Biotechnology > 97 > 6 > 1353 - 1362

Aqueous two‐phase systems (ATPS) have been demonstrated to be a simple and robust worldwide applied bioprocessing technology. From high value microscopic to macroscopic biological molecules obtained from a wide broad of matrices, ATPS have shown a high efficiency capacity for their concentration, enrichment, recovery, and purification. Particularly in Latin America, four relevant countries have paved...

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Advances, current challenges, and future trends in bioseparation: perspective analysis of the papers published in JCTB

Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 97 > 3 > 558 - 560

Bioseparation is a crucial process for the obtention of biological products that are essential in daily life, such as medicine and food. At first, the researchers centered their efforts on the optimization of the upstream processes, conducting to high product titer. Afterwards, they turned their attention to improving the downstream processes. Since then, a revolution of technologies has been reported...

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Stem cell culture media enriched with plant‐derived compounds: Cell differentiation enhancement

Karolina Chairez‐Cantu, Alonso Ornelas‐González, Margarita Ortiz‐Martínez, Mirna González‐González, więcej

Journal of Chemical Technology & Biotechnology > 96 > 10 > 2711 - 2720

The development of cell therapies to replace lost or damaged cells has attracted interest in the regenerative medicine field due to the multilineage differentiation ability of stem cells. However, there are some drawbacks regarding optimization in the differentiation protocols to obtain high efficiency and further translation into clinic settings, which have led to the identification of specific compounds...

artykuł

Cover Image, Volume 96, Issue 9

Alonso Ornelas‐González, Karolina Chairez‐Cantu, Margarita Ortiz‐Martínez, Mirna González‐González, więcej

Journal of Chemical Technology & Biotechnology > 96 > 9 > i - i

The cover image is based on the Review Stem cell culture media enriched with plant‐derived compounds: Cell proliferation enhancement by Alonso Ornelas‐González et al., https://doi.org/10.1002/jctb.6794.

artykuł

Stem cell culture media enriched with plant‐derived compounds: Cell proliferation enhancement

Alonso Ornelas‐González, Karolina Chairez‐Cantu, Margarita Ortiz‐Martínez, Mirna González‐González, więcej

Journal of Chemical Technology & Biotechnology > 96 > 9 > 2426 - 2435

Stem cells are characterized by their self‐renewal and differentiation potential, emerging as a promising strategy for developing cell therapies to treat degenerative diseases. Stem cell culture under in vitro conditions involves the addition of growth factors to the media to stimulate their proliferation. However, most of these growth factors are of animal origin, hindering the implementation of...

artykuł

Alternative acetone–water separation process through the application of aqueous two‐phase systems

José Omar García Gómez, Nancy Eloisa Rodríguez Olalde, Ana Alejandra Vargas Tah, Marco Rito‐Palomares, więcej

Journal of Chemical Technology & Biotechnology > 96 > 3 > 578 - 582

BACKGROUND The central stage of biotechnological production is the transformation of renewable raw materials into bioproducts in a water‐based bioreactor. The fermentation broth usually has a low concentration (less than 10%) of the bioproduct, which requires the application of subsequent separation and purification processes. For many bioproducts, separation is complicated by the presence of non‐ideal...

artykuł

Novel approach for neuronal stem cell differentiation using aqueous two‐phase systems in 3D cultures

Karolina Chairez‐Cantu, Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 96 > 1 > 8 - 13

Stem cell therapy has emerged as a promising alternative for replacing lost cells involved in neurodegenerative diseases. High efficiency of differentiation and full cell viability are actual challenges to achieve the translation of cell therapies to the clinic. To address this, the construction of aqueous two‐phase systems in three‐dimensional (ATPS‐3D) cultures has been proposed. This technique...

artykuł

Economic evaluation of M13 bacteriophage production at large‐Scale for therapeutic applications using aqueous Two‐Phase systems

Mario Torres‐Acosta, Alejandro González‐Mora, Federico Ruiz‐Ruiz, Marco Rito‐Palomares, więcej

Journal of Chemical Technology & Biotechnology > 95 > 11 > 2822 - 2833

BACKGROUND Bacteriophages are bionanoparticles with several applications in different biotechnology‐based products. Among them, vaccines have the potential to treat antibiotic‐resistant bacteria and parasitic infections. Traditional methods for their recovery and purification rely on precipitation with polyethylene glycol (PEG) and NaCl. However, the applicability of such an approach is limited,...

artykuł

Ribonuclease A modification with poly[N‐(2‐hydroxypropyl)methacrylamide] copolymers: new route of synthesis and purification

Calef Sánchez‐Trasviña, Karla Mayolo‐Deloisa, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 95 > 5 > 1321 - 1328

BACKGROUND N‐(2‐Hydroxypropyl)methacrylamide (HPMA), a synthetic polymer, has biocompatible features that allow it to be used as a drug carrier and used in drug delivery systems; however, its potential as a protein drug carrier has not been completely exploited. This study provides a new conjugation pathway to obtain HPMA–protein conjugates and, the use of hydrophobic interaction chromatography (HIC)...

artykuł

Cover Image, Volume 95, Issue 5

Calef Sánchez‐Trasviña, Karla Mayolo‐Deloisa, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 95 > 5 > i - i

The cover image is based on the Original Article Ribonuclease A modification with poly[N‐(2‐hydroxypropyl)methacrylamide] copolymers: new route of synthesis and purification by Marco Rito‐Palomares, Calef Sanchz‐Trasviña, and Karla Mayolo‐Deloisa., https://doi.org/10.1002/jctb.6367.

artykuł

Characterization and optimization of immunoaffinity aqueous two‐phase systems with PEGylated CD133/2‐biotin antibody in route to stem cell separation

Alonso Ornelas‐González, Stefanie U Reisenauer, Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 95 > 1 > 123 - 131

BACKGROUND A short processing time and efficient scale‐up stem cell isolation bioprocess is essential to exploit the potential of these cells for the treatment of multiple chronic diseases. Various methodologies have been used for stem cell recovery. However, most of them present economic and/or time‐consuming drawbacks. RESULTS This paper reports the characterization and optimization of immunoaffinity...

artykuł

Cell‐based aqueous two‐phase systems for therapeutics

Mirna González‐González, Marco Rito‐Palomares

Journal of Chemical Technology & Biotechnology > 95 > 1 > 8 - 10

Cell‐based aqueous two‐phase system technologies present an emerging and promising translational tool for the development of next‐generation therapeutic applications. Advantages such as biocompatibility, scalability and ease of implementation confirm this possibility. The aim of this perspective article is to present a brief, process‐oriented analysis of cell‐based aqueous two‐phase system strategies...

artykuł

Economic evaluation of the development of a phage therapy product for the control of Salmonella in poultry

Mario A. Torres‐Acosta, Viviana Clavijo, Christopher Vaglio, Andrés F. González‐Barrios, więcej

Biotechnology Progress > 35 > 5 > n/a - n/a

Poultry products are one of the major transmission media of Salmonella enteritidis to humans. A promising alternative to reduce the load of Salmonella in poultry are bacteriophages. Elsewhere, a mixture of six bacteriophages has been used successfully, but large‐scale production would be necessary to supply potential poultry market and costs analyses have not been calculated yet. For this, a powerful...

artykuł

Aqueous Two‐Phase Systems at Large Scale: Challenges and Opportunities

Mario A. Torres‐Acosta, Karla Mayolo‐Deloisa, José González‐Valdez, Marco Rito‐Palomares

Biotechnology Journal > 14 > 1 > n/a - n/a

Aqueous two‐phase systems (ATPS) have proved to be an efficient and integrative operation to enhance recovery of industrially relevant bioproducts. After ATPS discovery, a variety of works have been published regarding their scaling from 10 to 1000 L. Although ATPS have achieved high recovery and purity yields, there is still a gap between their bench‐scale use and potential industrial applications...

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