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β-Bungarotoxin (β-Bgt) consists of A chain (a phospholipase A 2 subunit) and B chain, cross-linked by an intersubunit disulfide bridge. In contrast to a marginal activity noted with β-Bgt, recombinant A1 chain and B1 chain markedly induced release of calcein from phospholipid vesicles. Reduction of intersubunit disulfide bond by dithiothreitol or glutathione enhanced membrane-damaging activity...
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