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AimsTo develop a qPCR approach for the detection of Pseudomonas aeruginosa in soil and manure and explore its efficacy and limitations compared with that of a classical culture‐dependent approach.
Methods and ResultsA Ps. aeruginosa ecfX qPCR assay was developed. This assay was optimized for soils of contrasting physico‐chemical properties and evidenced a three‐log dynamic range of detection [5 × 10...
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