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An assay for rapid and direct detection of DNA/mRNA using cationic fluorescent polymer based on one-dimensional microfluidic beads array (1-D chip) has been developed. The cationic water-soluble polythiophene derivatives can easily transduce hybridization events into measurable optical signal due to the conformational changes of the conjugated backbone, when mixed with single-stranded or double-stranded...
This study describes interaction mechanism between menadione and the yeast with the aid of the catabolite assay and the culture fluorescence method, with a view towards exploitting the potential of the mediator in toxicology study. Double mediator system containing menadione and the hydrophilic mediator, ferricyanide, was employed for evaluating the toxic effects of furfural on the yeast and the results...
2-(5-Bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) is a sensitive photometric reagent for determination of zirconium, when fosfomycin is added, it can quantitatively replace 5-Br-PADAP by complexing with zirconium, thus, an indirect spectrophotometric method based on ligand exchange has been established. The detection wavelength is at 605 nm, and the apparent molar absorption coefficient was...
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