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We describe a quick and reliable protocol to determine the plus or minus mating type of haploid Chlamydomonas reinhardtii strains from very small amounts of cells. The method combines a fast DNA preparation adapted from forensic work of Walsh et al. (1991) with one for use with Chlamydomonas by Berthold et al. (1993). We used PCR to amplify the minus-specific mid gene (minus dominance) or the plus...
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