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In an acidic medium, sodium dodecanesulfonate and acridine orange combine with human serum albumin (HSA) to form a macromolecular complex. This results in a significant enhancement of the resonance Rayleigh-scattering (RRS) intensity and a new strong peak at 397 nm, permitting highly sensitive determination of HSA. RRS intensity at 397 run is directly proportional to the concentration of HSA in the...
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