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Nitrous oxide reductase (N2OR) from Pseudomonas stutzeri catalyzes the final step in denitrification: the two‐electron reduction of nitrous oxide to molecular dinitrogen. Crystals of the enzyme were grown under strict exclusion of dioxygen by sitting‐drop vapour diffusion using 2R,3R‐butanediol as a cryoprotectant. N2OR crystallized in either space group P1 or P65. Interestingly, the key determinant...
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