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In the present study, Dictyostelium discoideum AX2-R2 was grown on living bacteria Escherichia coli B/r in shake-flasks and in standard stirred tank bioreactors. The maximum cell densities of D. discoideum detected in shake-flasks and in bioreactors were almost identical with 1.3×10 7 and 1.4×10 7 ml −1 , respectively. More than 99% of the bacterial biomass was consumed in...
Dictyostelium discoideum is a social amoeba. It shows promising characteristics as an expression system for the production of recombinant glycoproteins owing to its ability to perform a whole spectrum of post-translational modifications. However, its application is seriously affected by slow growth rates as well as low maximal cell densities. Since the technique of immobilization often is applied...
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