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Granulocyte colony stimulating factor (G‐CSF) is a cytokine used to treat neutropenia. Different glycosylated and non‐glycosylated variants of G‐CSF for therapeutic application are currently generated by recombinant expression. Here, we describe our approaches to establish a first semisynthesis strategy to access the aglycone and O‐glycoforms of G‐CSF, thereby enabling the preparation of selectively...
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