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A protocol for rapid in vitro-multiplication of Plumbago zeylanica L. through axillary bud proliferation was developed as an essential prerequisite to conduct genetic transformation studies. A maximum of 3.5 ± 0.5 shoots were produced from a single nodal segment of a four year old field grown plant after 4 weeks of transfer to Murashige and Skoog's (MS) basal medium supplemented with 8.87 mmol/L BAP...
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