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Objective: To examine the effects of different thermodynamic control systems on the temperature stability of human eggs during in vitro manipulation, with the integrity of meiotic spindles imaged using the LC-PolScope (Cambridge Research & Instrumentation, Inc., Woburn, MA).Design: We performed intracytoplasmic sperm injection (ICSI) and/or imaging of eggs with the temperature regulated by three...
Objective: To image spindles in living human oocytes and to examine the relation between spindles and fertilization after ICSI.Design: The LC polscope was used to examine spindles in an observational study of living oocytes.Setting: Academic IVF clinic.Patient(s): Women being treated for infertility.Intervention(s): Oocytes retrieved from patients for infertility treatment were examined before ICSI...
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