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The current study was designed to examine the role of galloyl moieties in the interaction of β-lactoglobulin (β-Lg) with (−)-epigallocatechin-3-gallate (EGCG) using fluorescence and CD spectroscopic methods. The interactions with β-Lg were investigated for EGCG, (−)-epigallocatechin (EGC), and the phenolic compound methyl gallate (MeG) at pH values 3.0, 5.0 and 7.4. The spectroscopic data indicated...
The effects of metal ions (Cu2+, Sn2+, Zn2+, Mn2+, Al3+ and Fe3+) on the interaction between (−)-epigallocatechin-3-gallate (EGCg) and β-lactoglobulin (β-Lg) were investigated via fluorescence and circular dichroism (CD) spectroscopy. The results of the fluorescence titration experiment demonstrated that the addition of Cu2+ increases the quenching efficiency of EGCg to β-Lg by more than EGCg alone,...
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