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In common fluorescence microscopy with one-photon excitations (OPE), the space resolution is about λ/2. Using femto-second (fs) laser, the two-photon excitation (TPE) can be achieved on the center part of the focused spot breaking the resolution limit producing the high resolution microscopy. In this work, the living cell imaging under OPE and TPE were comparatively measured. The autofluorescence...
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