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The effect of chemical activation protocols on in vitro-matured oocytes were compared to results with IVF (natural activation). Buffalo ovaries were collected in normal saline and transported to the laboratory within 2h of slaughter. Good quality oocytes, collected by aspiration from 3 to 10mm follicles, were matured for 22–24h. Matured oocytes were subjected to either IVF (control) or chemical activation...
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