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The aim of the study was to develop a procedure of purifying rabbit anti-rat TRβΔ polyclonal antibody from the antiserum. TRβΔ was a newly reported thyroid hormone receptor beta isoform. To gain better understanding of this new receptor, such as its tissue distribution, its specific antibody was needed. In this study, chemically synthesized peptide, which is uniquely existed in the TRβΔ, was used...
A novel thyroid hormone receptor isoform TR beta Delta was newly reported and deposited in Genbank(DQ191165.1) by our lab. To gain more understanding of this new variant. We raised rabbit polyclonal antibodies specifically against the characteristic epitope, amino acids 129-164 named as betaDelta36, of TRbetaDelta. Coding sequence for this betaDelta36 peptide was obtained by PCR amplification and...
Cartilage damage is difficult to repair,as a major stimulator of matrix synthesis in cartilage, insulin-like growth factor I (IGF-I) is likely to play a important role in the process of repairing cartilage damage. To study the in vivo therapeutic effect of chitosan mediate igf-lgene transfection on repairing articular cartilage defect in rabbits.Chitosan-DNA nanoparticlesCS/igf-1 were prepared following...
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