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In order to elucidate the mechanisms of the neuronal anti-opioid activity of Neuropeptide FF, we have transfected the SH-SY5Y neuroblastoma cell line, which expresses μ- and δ-opioid receptors, with the human NPFF 1 receptor. The SH 1 -C7 clone expresses high affinity NPFF 1 receptors in the same range order of density as opioid receptors. Similarly to the opioids, acute stimulation...
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