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An “off-on” phosphorescent aptasensor based on the 3-mercaptopropionic acid (MPA) capped Mn-doped ZnS quantum dots (MPA-Mn:ZnS QDs)/aptamer hybrid system was developed to detect adenosine triphosphate (ATP) in biological fluids. The phosphorescence of MPA-Mn:ZnS QDs was obviously quenched when ATP aptamer was added due to the aggregation induced effect. ATP aptamer, adsorbed on the surface of the...
Carbon monoxide is a critical gasotransmitter in the body and related with mitochondrial respiration. To date, various fluorescent probes for CO have been well proposed, but two main problems remain. One is that most of the probes are not mitochondria-targeting, even if the probes claim to be able to detect CO in living cells. The other is that the probes for CO display excitation and emission within...
A miniaturised high pressure 6-port injection valve has been designed and evaluated for its performance in order to facilitate the development of portable capillary high performance liquid chromatography (HPLC). The electrically actuated valve features a very small size (65 × 19 × 19mm) and light weight (33g), and therefore can be easily integrated in a miniaturised modular capillary LC system suited...
Rare-earth-doped upconversion nanoparticles (UCNPs) behaved advantages such as non-autofluorescence, environment-friendly synthetic route, low cost and simple detection procedures. In addition, the UCNPs show unique upconvering optical property of converting low-energy infrared photons to high‐energy visible ones. Here in our work, water and ethylene glycol at the ratio of 1/1 (v/v) were chosen as...
Metal ions differed greatly in affinity towards phosphopeptides, and thus it is essential to systematically compare the phosphopeptides enrichment ability of different metal ions usually used in the IMAC techniques. In this work, for the first time, eight metal ions, including Nb5+, Ti4+, Zr4+, Ga3+, Y3+, In3+, Ce4+, Fe3+, were immobilized on the polydopamine (PDA)-coated Fe3O4 (denoted as Fe3O4@PDA-M...
Highly efficient and selective enrichment of glycopeptides from complex biosamples is vital prior to mass spectrometry analysis. In this work, a hydrophilic metal organic framework with free carboxylic groups (denoted as UiO-66-COOH) was synthesized in one step with binary ligands. Compared with pure UiO-66 with one ligand, UiO-66-COOH containing binary ligands demonstrated greater hydrophilicity...
In this study, a new and sensitive signal-off electrogenerated chemiluminescence (ECL) biosensing method for the quantification of 5-hydroxymethylcytosine in DNA (5-hmC-DNA) was developed. The method achieved simple and sensitive detection of 5-hmC-DNA based on the glycosylation of 5-hmC, combining both the amplification function of gold nanoparticles (AuNPs) and the high quenching efficiency of the...
Flame retardants are associated to numerous adverse health effects, can accumulate in humans and have been used intensively worldwide. Recently, dust has been identified as a major human exposure route for flame retardants. The aim of this study was to develop a multi-residue method using a two-step SPE purification. It enabled us to effectively limit co-extracted matrix/interferets and therefore...
A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction...
We developed a novel, simple and fast method for the determination of glucocorticoids residues in liver tissue by combining microwave-assisted enzymatic hydrolysis and restricted access matrix dispersive solid phase extraction (RAM-dSPE) followed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Firstly, microwave-assisted enzymatic hydrolysis was introduced in order to obtain...
5nm intense green emission NaYF4:Yb/Er upconversion nanoparticles (UCNPs) with pure β phase was synthesized with a simple “green” strategy for the first time. Traditional organic solvothermal method is often applied to prepare the high-quality and uniform UCNPs, but the preparation of lanthanide-oleate complexes is laborious as heating and multistep post-treatment for purification are often required...
A disposable electrochemical immunosensor was developed for detection of aflatoxin B1 (AFB1) based on stripping voltammetric detection of copper ions released from Cu-apatite. AFB1 antibody (Ab) was firstly fixed on the gold nanoparticle (Au NPs) modified screen-printed carbon electrode (SPCE). AFB1-bovine serum albumin (AFB1-BSA) conjugate was labeled with Cu-apatite, and then competed with AFB1...
A ready-to-use l-ascorbic acid (l−AA) colorimetric strip based on polyaniline (PANI)/polyamide 66 (PA 66) nanofiber/net (NFN) membrane with spider-web-like structure was fabricated through a facile bend electrospinning/netting process. Introduction of PANI component into the membrane turns the strip undergoes a brilliant brown-to-green color transition within 30min upon incubation with l-AA. Moreover,...
Carbon dots capped with polyethyleneimine (CD-PEI) were synthesized and applied in selective separation and preconcentration of trace Cr(VI). Dispersed particle extraction (DPE) slurry sampling with flame atomic absorption spectrometry (FAAS) was used to selectively and sensitively determine Cr(VI) in water samples. The as-synthesized CD-PEI was confirmed by Fourier transform infrared spectroscopy,...
In this work, the competitive interaction between dibucaine and three fluorescent probes (i.e., berberine, palmatine, and coptisine) for occupancy of the cucurbit[7]uril (CB[7]) cavity was studied by fluorescence spectra, UV–visible absorption spectra, 1 H NMR spectra, and theoretical calculations in acidic aqueous solution. Based on the fluorescence enhancement of berberine, palmatine, and...
Interest in carbon nanotubes for detecting the presence of pathogens arises because of developments in chemical vapor deposition synthesis and progresses in biomolecular modification. Here we reported the facile synthesis of multi-walled carbon nanotubes (MWCNTs), which functioned as immuno-, magnetic, fluorescent sensors in detecting Vibrio alginolyticus (Va). The structures and properties of functionalized...
Pillar[5]arenes bearing ten phosphine oxide groups (1a–e), as analogs of their corresponding calix[4]arene-based phosphine oxide, have demonstrated intriguing recognition performance for some representative heavy metal cations including Co 2+ , Cu 2+ , Ni 2+ , Zn 2+ , Cd 2+ , Pb 2+ , Ag 2+ and Hg 2+ compared to their acyclic species (2a–e)...
Fluorescence analysis by means of a single fluorescence signal output usually leads to the signal fluctuation caused by various external factors. Ratiometric fluorescence probes that can significantly eliminate the external effects by self-calibration of two different emission bands are preferable for the detection of real samples. In this work, we designed a dual-emission quantum dots (QDs) nanocomposite...
Hydrogen peroxide biosensor based on the silica cavity array modified indium-doped tin oxide (ITO) electrode was constructed. An array of silica microcavities was fabricated by electrodeposition using the assembled polystyrene particles as template. Due to the resistance gradient of the silica cavity structure, the silica cavity exhibits a confinement effect on the electrochemical reactions, making...
In this study, a novel enrichment technique based on magnetic core-mesoporous shell microspheres with C8-modified interior pore-walls (C8-Fe 3 O 4 @mSiO 2 ) was successfully developed for the determination of diazepam in rat plasma by LC-MS. Due to the unique properties of the synthesized C8-Fe 3 O 4 @mSiO 2 microspheres (C8-modified magnetic mesoporous...
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