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We have studied interactions between positively charged MUTAB‐stabilized quantum dots (QDs) and model proteins, serum and live cells using fluorescence correlation spectroscopy (FCS), dynamic light scattering (DLS), time‐resolved photoluminescence (PL) and live‐cell fluorescence imaging. Using human serum albumin (HSA) as a model protein, we measured the growth of a protein adsorption layer (“protein...
Nanoparticle (NP) interactions with cells and organisms are mediated by a biomolecular adsorption layer, the so‐called “protein corona.” An in‐depth understanding of the corona is a prerequisite to successful and safe application of NPs in biology and medicine. In this work, earlier in situ investigations on small NPs are extended to large polystyrene (PS) NPs of up to 100 nm diameter, using human...
Biological responses of cells and organisms to nanoparticle exposure crucially depend on the properties of the protein adsorption layer (“protein corona”) forming on nanoparticle surfaces and their characterization is a crucial step toward a deep, mechanistic understanding of their build‐up. Previously, adsorption of one type of model protein on nanoparticles was systematically studied in situ by...
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