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Myc-RP from Perilla frutescens and Delila from Antirrhinum majus, two plant basic helix–loop–helix transcription factors (bHLH TFs) involved in the flavonoid biosynthetic pathway, have been used for the improvement of transactivational properties by directed evolution. Through two rounds of DNA shuffling, Myc-RP variants with up to 70-fold increase in transcriptional activities have been identified...
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