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Three different genes named sn311, sn316 and sn285 were discovered by large-scale randomly sequencing the high quality cDNA library of the venom glands from Hydrophiinae Hydrophis cyanocinctus Daudin. Sequence analysis showed that these three genes encoded three different short chain α-neurotoxins of 81 amino acids, which contained a signal peptide of 21 amino acids and followed by a mature peptide...
A full-length cDNA of neurotoxin (Hk2a) was isolated by RT-PCR of total RNA isolated from tentacles of Anthopleura sp. using degenerate oligonucleotide primers and 3',5'-RACE. The cDNA sequence of Hk2a encoded a polypeptide of 47 amino acids, which lacks a typical N-terminal signal sequences commonly found in proteins that are secreted via endoplasmic reticulum-Golgi pathway, indicating the possibility...
Three full-length phospholipase A 2 (PLA 2 ) cDNAs from sea snake Lapemis hardwickii venom were cloned and sequenced in our previous study. In order to investigate their biological functions, we established a fusion expression system for PLA 2 -9 in E. coli. The open reading frame encoding mature peptide of PLA 2 -9 was subcloned into the vector pTRX. The Trx-PLA ...
A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro...
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