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Cotton fibers are single-cell trichomes derived from the outer integument cells of ovules. Recent studies showed that the rapid elongation of fibers was coordinated with the expression of a subset of fiber-specific genes. Here we report the use of filter arrays of cDNAs to identify genes preferentially expressed in cotton fibers. An array of 1536 clones was hybridized with cDNA probes prepared from...
A cDNA, GaPR-10, encoding a pathogenesis-related class 10 protein, was isolated from Gossypium arboreum, a diploid cotton species. The predicted protein of 159 amino acids contains a conserved domain (K-A-X-E-X-Y-L) in the C-terminal helix. The glycine-rich P-loop structure found in Betv1 [1] is variable in GaPR-10. The bacterially expressed GaPR-10 exhibited ribonuclease activity in vitro. Substitution...
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