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Gemcitabine (GEM) resistance affects chemotherapy efficacy of pancreatic cancer (PC). Cancer‐associated fibroblasts (CAFs) possess the ability of regulating chemoresistance. This study probed the mechanism of hypoxia‐treated CAFs regulating cell stemness and GEM resistance in PC. Miapaca‐2/SW1990 were co‐cultured with PC‐derived CAFs under normoxic/hypoxic conditions. Cell viability/self‐renewal ability...