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Yeast is a widely used host for recombinant protein expression. However, glycoproteins derived from yeast contain N-glycan of high mannose type and are usually hyperglycosylated. α-1,6-mannosyltransferases gene (och1) encodes the enzyme that initiate the first step of out-chain elongation of high mannose type N-glycan in yeast, which is different in humans. Hence, a highly efficient method to knockout...
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