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Exosomes have proved to be an effective cancer biomarker with significant potential, and several cell-specific molecules have been found in colorectal cancer (CRC) exosomes. Nevertheless, it is challenging to use exosomes in clinical lab diagnostics due to their nanoscale and the lack of a convenient and effective detection platform. Here, we developed a DNase I enzyme-aided fluorescence amplification...
A magnetic fluorescent nano-thermometer is presented. To fabricate the nano-thermometer, magnetic nanoparticles (Fe3O4) were first encapsulated with a silica layer. Then a poly (N-isopropylacrylamide) (pNIPAM) copolymer shell with Rhodamine B isothiocyanate (RhBITC) embedded inside was further coated, which was denoted as the pNIPAM-co-RhBITC shell. Finally, gold nanoparticles were introduced onto...
Here, we report on a novel nonenzymatic amperometric glucose sensor based on CuO nanoneedle/graphene/carbon nanofiber modified electrode. The results of the scanning electron microscopy indicate that electronic network was formed through their direct binding with the graphene/carbon nanofiber, which leads to larger active surface areas and faster electron transfer for the glucose sensor. High electrocatalytic...
A magnetically controllable dual-mode optical probe is demonstrated for cellular imaging with an onion-liked structure, which can exhibit both surface enhanced Raman scattering (SERS) and fluorescence signals. For obtaining such a nanoprobe, Fe 3 O 4 nanoparticles were first encapsulated into an inner layer of silica, which were then coated with a second layer of gold nanoshell (designated...
Multifunctional fluorescent silica nanoparticles with phenylboronic acid tags were developed for labeling sialic acid on the surface of living cancer cells. In this paper, fluorescent silica nanoparticles (FSNPs) with strong and stable emission at 515nm were firstly prepared through a reverse microemulsion process, and then modified with highly selective phenylboronic acid (PBA) tags on their surface...
A novel microfluidic chip coupled with near-infrared (NIR) fluorescence detection set-up was established for specific analysis of trace Hg 2+ . Based on the NIR fluorescence changes caused by Hg 2+ -induced dissolution and aggregation of label-free single-strand DNA (ssDNA) wrapped single-walled carbon nanotubes (SWNTs), Hg 2+ in aqueous solution were detected sensitively and...
A method to study Abl1tyrosine kinase inhibitors (TKIs) by liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) was developed and validated. Chromatographic separation was achieved on a Symmetry ® C-18 column using a gradient. The detection was performed by selected ion monitoring (SIM) mode via positive ESI interface. The limit of quantification (LOQ) was 40.8nM for...
In the present work, new water-soluble fluorescent carbon nanodots (C-dots) were prepared in a facile microwave pyrolysis approach in minutes by combining glycine and polyethylene glycol 200 (PEG 200). Transmission electron microscopy (TEM) measurements showed that the resulting C-dots had diameters of about 3nm. 13 C NMR spectra further confirmed the presence of carbons (sp 2 and...
Hydrophilic carbon-functionalized magnetic microspheres coated with chitosan (Fe 3 O 4 @C@CHI) were synthesized for the first time. The hydrophilic carbonaceous layer on the Fe 3 O 4 microspheres was formed by the hydrothermal reaction of Fe 3 O 4 microspheres and glucose, and then chitosan was coated on surface of the carbon-functionalized microspheres...
A kind of fluorescent dye-doped, silica-coated Au aggregates was fabricated using reverse microemulsion method, which shows both strong fluorescence and intense surface enhanced Raman scattering (SERS) signals. Such a composite nanoparticle is composed of a SERS core as silica-coated Au aggregates and a fluorescent shell as dye-doped silica shell. Being prepared through reverse microemulsion method,...
A novel fluorescent Au@SiO 2 nanocomposite, with average size of ca. 30nm in the diameter, was prepared via a simple microemulsion method. Additionally, transmission electron microscopy (TEM), UV–Vis absorption spectra, Fourier transform infrared (FT-IR) spectra and fluorescence spectra were used to characterize this nanocomposite. This newly synthesized, silica-wrapped, gold nanocluster has...
In this work, a micro-plate chemiluminescence enzyme immunoassay by antibody-coated for the determination of aflatoxin B1 (AFB1) in agricultural products has been established. Aflatoxin B1 antibody (AFB1-Ab) was adsorbed physically on polystyrene micro-plate hole as solid phase antibody, which took place immunity-reaction between antigen and antibody with AFB1 standard solution or samples by direct...
A competitive immunoassay for estradiol (E2) based on secondary antibody format was established. The donkey anti-rabbit IgG was used as the secondary antibody to coat micro-plates, and the horseradish peroxidase (HRP)–luminol–H 2 O 2 chemiluminescent system with high sensitivity was chosen as the detection system. The addition of sodium trichloroacetate (CCl 3 COONa) in the...
In the present work, a strongly chemiluminescence (CL) from the mixing of hydrogen peroxide (H 2 O 2 ), hydrogen carbonate (HCO 3− ) and CdSe/CdS quantum dots (QDs) was observed. The addition of trace amount of l-ascorbic acid into the CL system caused significant quenching luminescence intensity. Based on this phenomenon, a flow injection CL analysis system for the determination...
Micro-total analysis systems (μTAS) integrate different analytical operations like sample preparation, separation and detection into a single microfabricated device. With the outstanding advantages of low cost, satisfactory analytical efficiency and flexibility in design, highly integrated and miniaturized devices from the concept of μTAS have gained widespread applications, especially in biochemical...
This paper describes an immunomagnetic separation of target bacterial cells from others by using magnetic bead. The surface of bead was coated with antibodies which can capture specific organism. The binding efficiency of immunomagnetic bead (IMB) capturing target bacterial cells was higher than 98% when the concentrations of target and interferent bacterial cells were at the same level. The concentration...
A highly sensitive and reproducible lead sensor based on a cyclodextrin-modified gold electrode was created. A self-assembled monolayer (SAM) of thiolated β-cyclodextrin (6-(2-mercapto-ethylamino)-6-deoxy-β-cyclodextrin (MEA-β-CD)) was prepared and modified on a gold electrode (MCGE) for specific Pb 2+ -sensing. Thus the mercury-free sensors for Pb 2+ assay based on MCGE were established...
A method to quantitatively perform reverse transcription-competitive PCR (RT-cPCR) of hepatitis C virus followed by both microchip and capillary electrophoretic separation and detection was described. In this method, HCV wild-type (WT) RNA extracted from serum was coretrotranscribed and coamplified with a constant amount of recombinant internal standard (IS) RNA which had the same primer binding region...
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