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We used an electrical cell-substrate impedance sensing (ECIS) technique to measure changes in the projected area of individual cells. The projected area of the cell plays a significant role in cellular behavior, including cell spreading, cell growth, cell death and cell adhesion. We built a custom impedance sensing setup; we recorded real time impedance spectra of individual adherent cells; and we...
Automation is essential for the high-throughput capture and analysis of single cells. To study multiple aging cells in parallel, the cell must be automatically trapped with micro-technologies and held while it divides over its lifetime. In this paper, progress toward a system that will automate single-cell yeast pedigree analysis is presented
A proof-of-platform-concept architecture for the analysis of living cell arrays is presented. Initial experiments conducted show a conceptual design for single-cell multiparameter measurements in real time. As a first parameter, extensive development and experimentation have been conducted on a sensor that will measure single-cell oxygen consumption. Wafer-level processing for chip fabrication and...
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