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Recent studies have demonstrated that the expression of aquaporin1 (AQP1) in endothelia cells played important roles in cell migration, and migration-associated cell function such as wound healing, and neutrophil motility. In the present study, we provided evidence that AQP1 expression in vascular endothelia cells (VECs) during angiogenesis was associated with MRTF-A. Morphology and immunofluorescence...
Recent studies uncovered an important role of aquaporins in cell migration, and migration-associated cell function such as angiogenesis, wound healing, and neutrophil motility. Here we provide evidence that aquaporin expression is involved in MCF-7 human mammary carcinoma cell migration. Reverse transcription-polymerase chain reaction, immunoblot and immunofluorescence analysis demonstrated two populations...
Bone marrow mesenchymal stem cells (MSCs) can differentiate into smooth muscle cells (SMCs) and have tremendous potential for cell therapy and tissue engineering. In this study, to understand the effects of TGF-beta3 on rat bone marrow-derived MSCs and the underlying molecular mechanism of this differentiation process, we investigated that the changes of myocardin-related transcription factors (MRTFs)...
Myocardin-related transcription factors A (MRTF-A) is a myocardin-related transcription factor that has been found strongly activated CarG box - containing genes through its direct binding to serum response factor (SRF). In the present study, the MRTF-A expression vector was constructed. The MTT assay showed that transfection of MRTF-A could significantly decrease the anti-tumor effect of tamoxifen...
Recent studies have demonstrated that aquaporin (AQP) expression facilitates cell migration and promotes angiogenesis and neutrophil motility. Migration of tumor cells is a crucial step in tumor invasion and metastasis. In the present study, we investigated the expression of AQP in human W489 colon cancer cells and characterized its function in cell migration. Two populations of W489 cell clones with...
Promoter fragment of alkaline protease gene was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. The fragment was sequenced and analyzed, then submitted to GenBank (EU130686). The results showed that it contained several typical promoter charactered regions and two reverse translation frames located in - 538 to - 370 bp and - 275 to - 128 bp region. Deletion analysis of the sequence indicated...
Aquaporin (AQP) has been reported to express in microvessels of many different type of tumor, indicating the possible involvement of AQP in tumor angiogenesis. Tumor angiogenesis is a mandatory step of tumor growth involving endothelial cell proliferation and migration from blood vessels in peritumoral tissues and formation of tubules. A murine Hepal-6 hepatocellular carcinoma-bearing model in AQP1...
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