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Streptothricins (STNs) are atypical aminoglycosides containing a rare carbamoylated D‐gulosamine (D‐GulN) moiety, and the antimicrobial activity of STNs has been exploited for crop protection. Herein, the biosynthetic pathway of the carbamoylated D‐GulN moiety was delineated. An N‐acetyl‐D‐galactosamine is first attached to the streptolidine lactam by the glycosyltransferse StnG and then epimerized...
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