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L-homoserine as a nonessential amino acid, can be used as a feed additive due to its significant value as well as in many other applications in agriculture. In this study, Escherichia coli W3110 was engineered for the production of L-homoserine from glucose. The lysA, metA, and thrBC genes were inactivated to block the competing and degradation pathways of L-homoserine. To enhance the carbon flux...
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