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NMR is one of the most versatile techniques for the analysis of molecular interactions. In this review we cover the main techniques and applications of the ligand‐observed methods: transfer‐NOESY, Saturation Transfer Difference NMR and Diffusion NMR. They have in common that monitoring the signals of the small molecule, used in high excess, allowed to obtain information about the ligand within the...
Background: Many plants respond to pathogenic attack by producing defense proteins that are capable of reversible binding to chitin, a polysaccharide present in the cell wall of fungi and the exoskeleton of insects. Most of these chitin-binding proteins include a common structural motif of 30 to 43 residues organized around a conserved four-disulfide core, known as the ‘hevein domain’ or ‘chitin-binding’...
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