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When maize coleoptiles were unilaterally exposed to red light (7.9 μmol m−2s−1 for 5 min), 3 h after treatment IAA levels in coleoptiles decreased in all regions, from top to basal, with levels about 60% of dark controls. Localized irradiation in the 5 mm top zone was sufficient to cause the same extent of IAA reduction in the tips to that in the tips of whole irradiated shoots. When coleoptiles were...
Coleoptile tips are the most likely candidates for the site of auxin (indole-3-acetic acid (IAA)) biosynthesis. Using a rapid, micro-scale method for IAA determination by gas chromatography–mass spectrometry, we investigated in detail the distribution and movement of free and conjugated IAA in maize (Zea mays L.) coleoptiles. The highest concentrations of IAA (approximately 250ng/g fresh weight) were...
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