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The mouse metallothionein (mMT) mutant α-KKS-α has a higher capacity for binding heavy metals than wild type mMT. The mMT mutant α-KKS-α gene was placed under the control of the strong promoter PpbsA to generate the intermediate vector pRL-α-KKS-α. pRLα-KKS-α was then linked with the plasmid pDC-08 to construct shuttle expression vector pDC-αKKS-α. This expression vector was transformed into Anabaena...
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