Search results for: Fengxia Lu

Items from 1 to 20 out of 71 results

article

Fermentation of millet bran with Bacillus natto: enhancement of bioactivity levels and the bioactivity of bran extract

Shimei Zhang, An Wang, Zhaoxin Lu, Fengxia Lu, more

Journal of the Science of Food and Agriculture > 104 > 10 > 6196 - 6207

BACKGROUND Millet bran (MB), a byproduct of millet production, is rich in functional components but it is underutilized. In recent years, researchers have shown that fermentation can improve the biological activity of cereals and their byproducts. This study used Bacillus natto to ferment millet bran to improve its added value and broaden the application of MB. The bioactive component content, physicochemical...

article

Blue light‐mediated gene expression as a promising strategy to reduce antibiotic resistance in Escherichia coli

Qingwei Jiang, Feng Geng, Juan Shen, Ping Zhu, more

Biotechnology Journal > 19 > 5 > n/a - n/a

The discovery of antibiotics has noticeably promoted the development of human civilization; however, antibiotic resistance in bacteria caused by abusing and overusing greatly challenges human health and food safety. Considering the worsening situation, it is an urgent demand to develop emerging nontraditional technologies or methods to address this issue. With the expanding of synthetic biology, optogenetics...

article

An endolysin Salmcide‐p1 from bacteriophage fmb‐p1 against gram‐negative bacteria

Ting Nie, Fanqiang Meng, Fengxia Lu, Xiaomei Bie, more

Journal of Applied Microbiology > 133 > 3 > 1597 - 1609

Aims A novel endolysin Salmcide‐p1 was developed as a promising candidate of new preservative and a supplement to effective enzyme preparations against gram‐negative bacterial contaminations. Methods and Results Salmcide‐p1 was identified by complementing the genomic sequence of a virulent Salmonella phage fmb‐p1. Salmcide‐p1 of 112 μg ml⁻¹ could quickly kill Salmonella incubated with 100 mmol...

article

Acetate and auto‐inducing peptide are independent triggers of quorum sensing in Lactobacillus plantarum

Fanqiang Meng, Fengxia Lu, Hechao Du, Ting Nie, more

Molecular Microbiology > 116 > 1 > 298 - 310

The synthesis of plantaricin in Lactobacillus plantarum is regulated by quorum sensing. However, the nature of the extra‐cytoplasmic (EC) sensing domain of the histidine kinase (PlnB1) and the ability to recognize the auto‐inducing peptide PlnA1 is not known. We demonstrate the key motif Ile‐Ser‐Met‐Leu of auto‐inducing peptide PlnA1 binds to the hydrophobic region Phe‐Ala‐Ser‐Gln‐Phe of EC loop 2...

article

Preparation of chitosan/curdlan/carboxymethyl cellulose blended film and its characterization

Keqin Wang, Lianchao Du, Chong Zhang, Zhaoxin Lu, more

Journal of Food Science and Technology > 2019 > 56 > 12 > 5396-5404

In this study, to improve the thermal and mechanical properties of chitosan films, a chitosan/curdlan/carboxymethyl cellulose (CS/CD/CMC) ternary blended film was prepared and characterized. To prepare a uniform CS/CD/CMC ternary blended film, an effective method of blending CD with other materials was established as the following conditions: the ternary solution temperature was maintained at 60 °C,...

article

Bacillomycin D inhibits growth of Rhizopus stolonifer and induces defense-related mechanism in cherry tomato

Fuxing Lin, Yingying Xue, Zhenghua Huang, Mengxi Jiang, more

Applied Microbiology and Biotechnology > 2019 > 103 > 18 > 7663-7674

The inhibitory effect of Bacillomycin D, a cyclic lipopeptide, on Rhizopus stolonifer colonization of cherry tomato was studied, and its possible mechanism of action was explored. Bacillomycin D showed a direct inhibitory effect on R. stolonifer spore germination and mycelial growth in vitro. It conferred both a direct inhibitory effect on R. stolonifer growth in cherry tomato in vivo and induced...

article

Influence of Backlash on Load Sharing and Dynamic Load Characteristics of Twice Split Torque Transmission System

Guanghu Jin, Wei Ren, Rupeng Zhu, Fengxia Lu

Journal of Vibration Engineering & Technologies > 2019 > 7 > 6 > 565-577

Introduction Based on the characteristics of the helicopter main reduction gearbox, and combined with the advantages of face gear and cylindrical gear power dividing, a new configuration of helicopter main gearbox with twice split paths is proposed. A bending–torsional coupled dynamic model of the system is developed through the lumped parameter method, and the influence of stiffness, damping and...

article

Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

Hui Qian, Chong Zhang, Zhaoxin Lu, Bingjie Xia, more

BMC Biotechnology > 2018 > 18 > 1 > 1-7

Background Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties. However, industrial applications of LOXs have been limited by their poor thermostability. Herein, we report a bioinformatics-based consensus concept approach for the engineering of thermostable Ana-rLOX. Results A...

article

Whey protein isolate with improved film properties through cross‐linking catalyzed by small laccase from Streptomyces coelicolor

Wei Quan, Chong Zhang, Meixia Zheng, Zhaoxin Lu, more

Journal of the Science of Food and Agriculture > 98 > 10 > 3843 - 3850

BACKGROUND The effects of small laccase (SLAC) from Streptomyces coelicolor on the properties of whey protein isolate (WPI) films were studied. RESULTS WPI was catalyze by SLAC without phenolic acid assistance. Particle size distribution results showed that some complexes with higher relative molecular weight formed in WPI samples treated with SLAC. The content of α‐helixes decreased while those...

article

Protective effects of Lactococcus lactis expressing alcohol dehydrogenase and acetaldehyde dehydrogenase on acute alcoholic liver injury in mice

Yunbin Lyu, Lei Zhong, Yanan Liu, Jing Lu, more

Journal of Chemical Technology & Biotechnology > 93 > 5 > 1502 - 1510

BACKGROUND Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) play important roles in alcohol metabolism. Therefore, a possible effective way to attenuate alcoholic liver damage is the exogenous supply of these two enzymes to the stomach as they might accelerate the oxidation of ethanol into nontoxic acetate. RESULTS ADH and ALDH were coexpressed in Lactococcus lactis NZ3900 and...

article

A facile method of preparing LiMnPO4/reduced graphene oxide aerogel as cathodic material for aqueous lithium-ion hybrid supercapacitors

Lin Xu, Senlin Wang, Xiao Zhang, Taobin He, more

Applied Surface Science > 2018 > 428 > C > 977-985

A facile method of preparing LiMnPO₄/reduced graphene oxide aerogel (LMP/rGO) as cathodic material was reported here. LiMnPO₄ nano-particles were prepared using a facile polyvinyl pyrrolidone-assisted solvothermal route. Then LMP/rGO aerogel was prepared using the accessible restacking method. The influence of the cathodic electrode composition (ratio of rGO to LiMnPO₄) on the performance of the LMP/rGO...

article

Growth inhibition of Fusarium graminearum and reduction of deoxynivalenol production in wheat grain by bacillomycin D

Jing Sun, Wei Li, Yanan Liu, Fuxing Lin, more

Journal of Stored Products Research > 2018 > 75 > C > 21-28

Fusarium graminearum causes Fusarium head blight, which leads to the quality and yield loss of wheat. In the present work, the inhibition effects of bacillomycin D (BD) on the growth of F. graminearum and reduction of deoxynivalenol (DON) production in wheat grain storage were investigated. The hyphal growth and sporulation of F. graminearum were restrained dramatically in the presence of 75 μg/mL...

article

Controllable synthesis of cross-linked CoAl-LDH/NiCo2S4 sheets for high performance asymmetric supercapacitors

Xiao Zhang, Senlin Wang, Lin Xu, Taobin He, more

Ceramics International > 2017 > 43 > 16 > 14168- 14175

Cross-linked CoAl-LDH/NiCo₂S₄ sheets hybrids hold a promising electrochemical material for high-performance supercapacitors. In this paper, we report a simple strategy for fabricating flocculent NiCo₂S₄ decorated on CoAl-LDH nanosheets to form integrated hybrid architectures (CoAl-LDH/NiCo₂S₄) via hydrothermal and electrodeposition method. Benefiting from the integrated structures of CoAl-LDH/NiCo...

article

Expression, purification, and characterization of a novel acidic Lipoxygenase from Myxococcus xanthus

Hui Qian, Bingjie Xia, Yujun He, Zhaoxin Lu, more

Protein Expression and Purification > 2017 > 138 > C > 13-17

The gene encoding a novel acidic lipoxygenase from Myxococcus xanthus DK1622 (accession: WP_011551853.1) was cloned into vector pET-28a and expressed in Escherichia coli BL21(DE3). The recombinant enzyme (rMxLOX), with a molecular weight of approximately 80 kDa, was purified to homogeneity using one-step nickel-affinity chromatography and showed an activity of 5.6 × 10⁴ U/mg. The optimum pH and temperature...

article

L-glutamine efficiently stimulates biosynthesis of bacillomycin D in Bacillus subtilis fmbJ

Shiquan Qian, Jing Sun, Hedong Lu, Fengxia Lu, more

Process Biochemistry > 2017 > 58 > C > 224-229

The present study investigated the stimulation effect of L-glutamine (L-gln) on bacillomycin D biosynthesis of Bacillus subtilis fmbJ and the transcription of the synthetase genes and the signal proteins that related to the biosynthesis of bacillomycin D. It was found that L-gln significantly increased the production of bacillomycin D, in comparison with other amino acids. L-gln significantly enhanced...

article

Characterization of a broad host-spectrum virulent Salmonella bacteriophage fmb-p1 and its application on duck meat

Changbao Wang, Qiming Chen, Chong Zhang, Jie Yang, more

Virus Research > 2017 > 236 > C > 14-23

The aim of this study was to find a virulent bacteriophage for the biocontrol of Salmonella in duck meat. A broad host-spectrum virulent phage, fmb-p1, was isolated and purified from an duck farm, and its host range was determined to include S. Typhimurium, S. Enteritidis, S. Saintpaul, S. Agona, S. Miami, S. Anatum, S. Heidelberg and S. Paratyphi-C. Electron microscopy and genome sequencing showed...

article

Heterologous production of a temperature and pH-stable laccase from Bacillus vallismortis fmb-103 in Escherichia coli and its application

Jianna Sun, Meixia Zheng, Zhaoxin Lu, Fengxia Lu, more

Process Biochemistry > 2017 > 55 > C > 77-84

To enhance laccase yield, the laccase gene from Bacillus vallismortis fmb-103 was cloned and heterologously expressed in Escherichia coli BL21 (DE3) cells. The auto-induction strategy was applied during fermentation, and the process was controlled, as follows: Cu²⁺ was added when the optical density at 600nm (OD₆₀₀) was 0.3, the fermentation temperature was adjusted to 16°C when the OD₆₀₀ was 0.9,...

article

Investigation on prevalence of Listeria spp. and Listeria monocytogenes in animal-derived foods by multiplex PCR assay targeting novel genes

Tingting Tao, Qiming Chen, Xiaomei Bie, Fengxia Lu, more

Food Control > 2017 > 73 > PB > 704-711

Chilled and frozen animal-derived food can be contaminated by Listeria spp., emerging foodborne pathogens in food industry. The objective of this study was to mine novel target genes by comparative genomics approach for multiplex PCR detection and differentiation of Listeria monocytogenes and other Listeria spp. in food. Multiplex PCR assay targeting the genetic markers LMOf2365_2721, AX25_00730,...

article

Development and application of a sensitive, rapid, and reliable immunomagnetic separation-PCR detection method for Cronobacter spp.

Qiming Chen, Yuanhong Li, Tingting Tao, Xiaomei Bie, more

Journal of Dairy Science > 2017 > 100 > 2 > 961-969

Cronobacter spp. have been linked to clinical cases of infection in both adults and infants. Enrichment of Cronobacter spp. before detection has been necessary but is quite time consuming. Hence, we sought to develop an immunomagnetic separation (IMS) PCR method that could shorten the time of enrichment before the detection of Cronobacter spp. The polyclonal antibody used in this immunomagnetic separation...

article

Functional Analysis of Plantaricin E and Its Mutant by Heterologous Expression in Escherichia coli

Fanqiang Meng, Xiaoyu Zhu, Fengxia Lu, Xiaomei Bie, more

Applied Biochemistry and Biotechnology > 2017 > 182 > 1 > 311-323

Plantaricins are a group of ribosomally synthesized antimicrobial peptides in Lactobacillus plantarum that exert antimicrobial activities against some foodborne pathogens. In this study, we observed that plantaricin E in L. plantarum 163 was missing 19 amino acids (plnE mutant amino acid sequence: FNRGGYNFGKSVRH, plnE amino acid sequence: FNRGGYNFGKSVRHVVDAIGSVAGIRGILKSIR). In order to study the effects...

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INFONA - science communication portal

Search results for: Fengxia Lu

Fermentation of millet bran with Bacillus natto: enhancement of bioactivity levels and the bioactivity of bran extract

Blue light‐mediated gene expression as a promising strategy to reduce antibiotic resistance in Escherichia coli

An endolysin Salmcide‐p1 from bacteriophage fmb‐p1 against gram‐negative bacteria

Acetate and auto‐inducing peptide are independent triggers of quorum sensing in Lactobacillus plantarum

Preparation of chitosan/curdlan/carboxymethyl cellulose blended film and its characterization

Bacillomycin D inhibits growth of Rhizopus stolonifer and induces defense-related mechanism in cherry tomato

Influence of Backlash on Load Sharing and Dynamic Load Characteristics of Twice Split Torque Transmission System

Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

Whey protein isolate with improved film properties through cross‐linking catalyzed by small laccase from Streptomyces coelicolor

Protective effects of Lactococcus lactis expressing alcohol dehydrogenase and acetaldehyde dehydrogenase on acute alcoholic liver injury in mice

A facile method of preparing LiMnPO4/reduced graphene oxide aerogel as cathodic material for aqueous lithium-ion hybrid supercapacitors

Growth inhibition of Fusarium graminearum and reduction of deoxynivalenol production in wheat grain by bacillomycin D

Controllable synthesis of cross-linked CoAl-LDH/NiCo2S4 sheets for high performance asymmetric supercapacitors

Expression, purification, and characterization of a novel acidic Lipoxygenase from Myxococcus xanthus

L-glutamine efficiently stimulates biosynthesis of bacillomycin D in Bacillus subtilis fmbJ

Characterization of a broad host-spectrum virulent Salmonella bacteriophage fmb-p1 and its application on duck meat

Heterologous production of a temperature and pH-stable laccase from Bacillus vallismortis fmb-103 in Escherichia coli and its application

Investigation on prevalence of Listeria spp. and Listeria monocytogenes in animal-derived foods by multiplex PCR assay targeting novel genes

Development and application of a sensitive, rapid, and reliable immunomagnetic separation-PCR detection method for Cronobacter spp.

Functional Analysis of Plantaricin E and Its Mutant by Heterologous Expression in Escherichia coli

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