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Several approaches have been taken for these in vivo studies. In many studies, the use of semi-quantitative immuno-electron microscopy is the approach of choice. Endogenous opioid receptors display differential subcellular distributions with μ opioid receptor (MOPR) being mostly present on the plasma membrane and δ-opioid receptor (DOPR) and κ-opioid receptor (KOPR) having a significant intracellular...
By using a rat opioid receptor cDNA as a probe to screen a human brain cDNA library, we isolated a 4.0-kb clone (z115) which encompasses a major portion of a human κ opioid receptor (hkor), extending from the amino acid residue #6 to the 3 -untranslated region. The extreme 5 -region 232-bp fragment of z115 was used as a probe to screen a human genomic DNA library. A 1.6-kb fragment (d2) of one...
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