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In this work, inorganic boronate affinity monolith was prepared by in situ synthesis in 0.33mm i.d. stainless steel needle through sol-gel process using tetraethoxysilane and tetrabutyl orthotitanate as the co-precursors. The morphology, structure and composition of the monolith were characterized. In contrast to conventional boronate affinity materials, inorganic boric acid was used as affinity ligand...
This paper reported the coupling technique of Ag island film-enhanced fluorescence with rare earth co-luminescence effect of Tb–Gd–sodium dodecyl benzene sulfonate (SDBS)–protein system. While the collagen is used as the separator between Ag island film and the fluorophore because it not only can decrease the fluorescence of the blank, but also can promote the adsorption of other proteins and change...
In the present study a new version of nonlinear partial least-square method based on artificial neural network transformation (ANN-NLPLS) has been proposed. This algorithm firstly transforms the training descriptors into the hidden layer outputs using the universal nonlinear mapping carried by an artificial neural network, and then utilizes PLS to relate the outputs of the hidden layer to the bioactivities...
In ordinary multivariate calibration methods, when the calibration set is determined to build the model describing the relationship between the dependent variables and the predictor variables, each sample in the calibration set makes the same contribution to the model, where the difference of representativeness between the samples is ignored. In this paper, by introducing the concept of weighted sampling...
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