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The aim of this study was to verify the existence of a side population (SP) of cells in second-trimester amniotic fluid. Amniotic fluid samples (n = 35) were obtained, and the number and size of viable amniotic fluid cells (AFCs) were analyzed. Small AFCs (SAFCs) and large AFCs (LAFCs) were isolated using a sterile 10-μm pore size strainer. Hoechst 33342 dye exclusion assay, flow cytometry analysis,...
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