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An in vitro conifer ectomycorrhizal model system was developed to study changes in gene expression during the establishment of symbiotic relationship. Using the model system and the mRNA differential display technique, we have identified cDNA clones from Laccaria bicolor that appear to be involved in establishing and maintaining the symbiotic relationship between L. bicolor and red pine. These differentially...
Paxillus involutus was transformed using gold particle mediated gene transfer. Transformation was determined using the hygromycin B phosphotransferase gene (hph) as the selectable marker and the β-glucuronidase gene (GUS) as a reporter gene. Southern blot analysis confirmed that the vector DNA for both hph and GUS genes was integrated into the fungal genome. Variations in the number of multiple gene...
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