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Purpose To investigate the microstructure of endothelial keratoplasty grafts using two-photon optical microscopy. Methods Six endothelial keratoplasty grafts obtained from human donor corneoscleral tissues and prepared by submerged hydrodissection technique were imaged by two-photon optical microscopy. In each graft, two liquid bubbles were created in order to investigate the presence of a conserved...
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