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The fluorescence spectral behavior of interaction of erythrosine sodium (ES) and bovine serum albumin (BSA) was investigated in hydroxypropyl-β-cyclodextrin (HP-β-CD) medium at pH 5.8. The excitation and emission wavelengths were 527nm and 549nm, respectively. The fluorescence intensity of ES increased due to the formed inclusion complex of HP-β-CD and ES. But the fluorescence intensity of ES–HP-β-CD...
In this paper, a simple and sensitive flow injection analysis (FIA) for the determination of protein with spectroscopic probe was developed. This method was based on the investigation of the interaction of tetrachloride fluorescein (2,4,5,7-tetrachloro-3,6-fluorandiol)–bovine serum albumin (BSA), the coupling reaction of protein with tetrachloride fluorescein (TCFS) which was used as a spectroscopic...
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