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We hypothesized that quantitative MS/MS‐based proteomics at multiple time points, incorporating immunoenrichment prior to rapid microwave and magnetic (IM2) sample preparation, might enable correlation of the relative expression of CD47 and other low abundance proteins to disease progression in the experimental autoimmune encephalomyelitis (EAE) animal model of multiple sclerosis. To test our hypothesis,...
We hypothesized that quantitative MS/MS‐based proteomics at multiple time points, incorporating rapid microwave and magnetic (M2) sample preparation, could enable relative protein expression to be correlated to disease progression in the experimental autoimmune encephalomyelitis (EAE) animal model of multiple sclerosis. To test our hypothesis, microwave‐assisted reduction/alkylation/digestion of proteins...
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