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In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with...
In the past decades, regression approach was a main gene mapping method. For avoiding the QTL mapping to estimate the gene effects, the molecular marker regression was used to research the quantitative trait controlled by polygene under the condition of complete linkage between gene locus and marker. For the utilization of this method, the recombination influence on precision and accuracy estimating...
A hapten di(2-ethylhexyl) 4-aminophthalate (DEHAP) which possesses the structural feature of di(2-ethylhexyl) phthalate (DEHP) was synthesized via esterification and reduction with 4-nitrophthalic acid and 2-ethyl-1-hexanol. The product was confirmed by infrared absorption spectroscopy (IR), 1H nuclear magnetic resonance spectroscopy (1H NMR) and mass spectrometry (MS). Then glutaraldehyde was used...
Monocrotophos as the hapten was coupled the carrier proteins ovalbumin (OVA) and bovine serum albumin (BSA) by Glutaral Method to synthesis artificial antigen and coating antigen. The identifications of synthetic antigens were by ultraviolet absorption spectrum scan and SDS-PAGE methods. And then BALB/C mices were immunized with immunogen (hapten-OVA). The result showed that UV spectrogram of synthetic...
Identifying the type and estimating the effect of genes controlling the quantitative trait is very useful to animal selection, breed and livestock production. In traditional quantitative genetics, the gene effect causing the variation of quantitative trait is researched as a whole body. The molecular marker technique provided an approach to analysis the gene characteristic of single gene. For avoiding...
In the past, the gene effect causing the variation of quantitative trait was researched as a whole body. For avoiding the QTL mapping, the molecular marker regression was used to research the quantitative trait controlled by polygene. A general analysis model using molecular marker material was established for estimating the gene effect controlling quantitative trait, and the mathematical relation...
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