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The simultaneous 3D arrangement of the interphase centromeres of chromosomes 7, 11, and 17 in unstimulated human T-lymphocytes is analyzed. Using triple in situ hybridization in combination with optical sectioning and image processing, the identification of three pairs of centromeres in each nucleus and the assignment of 3D coordinates to each centromere are made. The homologous and heterologous centromere...
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