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Bicinchoninic acid (BCA) is widely used for determining the valence state of copper in biological systems and quantification of the total protein concentration (BCA assay). Despite its well‐known high selectivity of Cu(I) over Cu(II), the exact formation constants for Cu(I)(BCA)23− and Cu(II)(BCA)22− complexes remain uncertain. These uncertainties, affect the correct interpretations of the roles of...
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