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By use of PCR, the genes encoding d-carbamoylase and d-hydantoinase, responsible for the synthesis of d-p-hydroxyphenylglycine (d-HPG), were cloned separately from Agrobacterium radiobacter NRRL B11291 into Escherichia coli. d-HPG production was carried out with a mixed population consisting of various proportions of two recombinants producing individual enzymes. As a result, with a total amount of...
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