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Vitamin D intoxication was produced with oral doses of either vitamin D 3 or 25-hydroxyvitamin D 3 in CYP27B1 −/− (1α-hydroxylase knockout) and wild-type mice. These compounds were equally toxic in wild-type and the mutant mice. Since the null mutant mice are unable to produce 1,25-dihydroxyvitamin D, it is clear 1,25-dihydroxyvitamin D is not responsible for vitamin D intoxication...
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