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Feeding sucrose at 20 g l−1 on day 16 gave maximum paclitaxel production at 10 mg l−1 when Taxus chinensis in 5 l bioreactors. Paclitaxel accumulation was doubled by the cultivation of cells initially with dissolved O2 tension at 60% for 20 days followed by being at 20% for another 12 days in the bioreactor. Combination of these two strategies gave maximum paclitaxel production of 19 mg l−1 after...
Candida guilliermondii grew on the d- and l-isomers of arabinose, xylose, ribose and lyxose. Xylose reductase was extracted from xylose-grown cells and the enzyme was purified to homogeneity. The purified enzyme used NADPH as a cosubstrate, had a molecular weight of 36 000 Da and pI of 4.5. It reduced the d- and l-forms of all the above pentoses.
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