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One hundred and thirty unrelated Azorean individuals were randomly selected to study the frequencies of high-resolution HLA alleles and haplotypes in the Azorean (Terceira) population. HLA-A, -B, -Cw, -DRB1, -DQA1 and -DQB1 high-resolution genotyping was performed by polymerase chain reaction using commercial kits. HLA-E, -F and -G alleles, were genotyped by sequence-based typing. All loci were in...
The potent paralytic shellfish toxins (PSTs) produced by Gymnodinium catenatum have appeared irregularly since the onset in 1986 of a monitoring program aimed at preventing contaminated bivalves from the Portuguese coast to reaching the consumer. In years where high contamination levels were attained, sporadic episodes of human poisonings were also recorded, as in 1994. The reappearance of high contamination...
Foodborne outbreaks attributed to marine biotoxins were first reported in Portugal in 1946. A regular monitoring programme was implemented in 1986 for PSP, in 1987 for DSP and in 1996 for ASP. The gradual introduction of HPLC methodologies for DSP and PSP allowed a better understanding of toxin biotransformation by bivalves, supplying more selective and sensitive data than mouse bioassays. A comprehensive...
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