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To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4 × 10-5 mol/L, 1.8 × 10-5 mol/L and 2.6 × 10...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and...
The purpose of this article was to investigate the apoptosis effect of the n-butanol extract from Capparis spinosa L. (CSBE) on human Gastric Cancer cells SGC-7901. MTT assay was used to measure the inhibitory rate of CSBE Morphological changes of apoptosis were observed with fluorescence microscope. Flow cytometry was employed to analyse the apoptotic rate of CSBE on SGC-7901 cells. Laser confocal...
This article studied whether genetic toxicity of toluene diisocyanate was reversible. Kunming mice were exposed to TDI by respiratory tract for 14 days in 1/8LC50, 1/4LC50 and 1/2 LC50 dosage and then were feed normally for 14 days. The chromosomal aberrations and the content of RNA & DNA were detected by optical microscope and laser scanning confocal microscope. In 1/4LC50 and 1/2 LC50 group,...
To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsim) levels. Saponins of asparagus with different concentration was treated with HepG2 and SGC-7901 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect...
To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4times10-5mol/L, 1.8times10-5mol/L and 2.6times10...
To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice A tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured...
Laser scanning confoncal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells...
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