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Oxidation–reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently,...
Semen hyperviscosity delays the liquefaction of semen sample and is subjected to limited proteolysis by addition of α‐chymotrypsin to reduce the viscosity. α‐Chymotrypsin is a proteolytic enzyme involved in degradation of the proteins and polypeptides. Even though α‐chymotrypsin improves the handling of hyperviscous samples, its effect on the sperm proteins is not clear. This study was aimed to evaluate...
One of the challenges with the sperm DNA fragmentation results is the inconsistency and the large variability in the results obtained by different techniques. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay quantifies the incorporation of fluoresceinated dUTP into single‐ and double‐strand DNA breaks by labeling the 3′‐OH terminal with TdT. The goal of this study was...
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