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Escherichia coli BL21(DE3) was engineered to divert the flow of carbon flux from glucose-1-phosphate to thymidine diphosphate 4-keto 4,6-dideoxy-D-glucose (dTKDG), an intermediate of various dTDP-sugars. Glucose phosphate isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf) and uridylyltransferase (galU) genes were deleted while two additional genes, dTDP-D-glucose synthase (tgs) and dTDP-D-glucose...
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