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In present study, 312, 554, 175 and 143 proteins were identified and quantified by label-free quantitative proteomics in human, cow, goat and yak milk fat globule membrane (MFGM), respectively. Fifty proteins involved in vesicle mediate transport and milk fat globule secretion were conserved among species. Moreover, proteins involved in lipid synthesis and secretion (xanthine dehydrogenase/oxidase,...
A novel protease, from the edible fungus Cordyceps sinensis, was purified and characterised. Its cleavage site motifs were determined by oriented peptide library mixtures and validated by synthetic peptides and natural proteins.The protease was purified to homogeneity using anion-exchange chromatography, sieve chromatography, native PAGE and reversion phase chromatography. Its molecular weight, estimated...
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